quickchange (New England Biolabs)
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New England Biolabs
quickchange
Quickchange, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickchange/Q5+Site-Directed+Mutagenesis+Kit/pm41680142-391-15-7
Average 99 stars, based on 5776 article reviews
Quickchange, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickchange/Q5+Site-Directed+Mutagenesis+Kit/pm41680142-391-15-7
Average 99 stars, based on 5776 article reviews
quickchange - by Bioz Stars,
2026-10
99/100 stars
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Generated:Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head-bound state. Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) (57, 58) or via Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head–bound state Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) ( , ) or via Article Title: The net charge of the K-loop regulates KIF1A superprocessivity by enhancing microtubule affinity in the one-head-bound state Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs) , or via Cloning:Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head-bound state. Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) (57, 58) or via Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head–bound state Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) ( , ) or via Article Title: The net charge of the K-loop regulates KIF1A superprocessivity by enhancing microtubule affinity in the one-head-bound state Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs) , or via Mutagenesis:Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head-bound state. Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) (57, 58) or via Article Title: Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus. Article Snippet: Following 1 h AR TI CL E IN P RE SS incubation at 50°C, the reactions were directly transformed into 50 μL of NEB 5-alpha or home-made competent top10 E. coli cells, by standard heat-shock procedures. .. For simpler nucleotide sequence changes, either the NEB Q5 site-directed mutagenesis kit or an alternative Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction Article Snippet: Constructs were cloned using Gibson assembly (SGI DNA, LaJolla CA), overlap extension PCR, and restriction endonuclease digest/ligation (Fermentas, Waltham MA). .. Site-directed mutagenesis was primarily performed using PCR with primer pairs designed using Article Title: Positive charge in the K-loop of the kinesin-3 motor KIF1A regulates superprocessivity by enhancing microtubule affinity in the one-head–bound state Article Snippet: .. Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly Cloning Kit from New England Biolabs) ( , ) or via Article Title: Limited Dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction Article Snippet: Constructs were cloned using Gibson assembly (SGI DNA, LaJolla CA), overlap extension PCR, and restriction endonuclease digest/ligation (Fermentas, Waltham MA). .. Site-directed mutagenesis was primarily performed using PCR with primer pairs designed using Polymerase Chain Reaction:Article Title: Revolutionising the design and analysis of protein engineering experiments using fractional factorial design Article Snippet: The reaction was carried out (Thermal Cycler, Bio-Rad, Hercules, USA) with primer annealing temperatures determined theoretically, and a long elongation time (30 seconds per kbp; 3.5 minutes). .. Following PCR the reactions were treated with either DpnI or a mixture of T4 DNA Ligase, T4 Polynucleotide Kinase and DpnI for the Article Title: Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction Article Snippet: Constructs were cloned using Gibson assembly (SGI DNA, LaJolla CA), overlap extension PCR, and restriction endonuclease digest/ligation (Fermentas, Waltham MA). .. Site-directed mutagenesis was primarily performed using PCR with primer pairs designed using Article Title: Limited Dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction Article Snippet: Constructs were cloned using Gibson assembly (SGI DNA, LaJolla CA), overlap extension PCR, and restriction endonuclease digest/ligation (Fermentas, Waltham MA). .. Site-directed mutagenesis was primarily performed using PCR with primer pairs designed using Sequencing:Article Title: Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus. Article Snippet: Following 1 h AR TI CL E IN P RE SS incubation at 50°C, the reactions were directly transformed into 50 μL of NEB 5-alpha or home-made competent top10 E. coli cells, by standard heat-shock procedures. .. For simpler nucleotide sequence changes, either the NEB Q5 site-directed mutagenesis kit or an alternative |